University of Dundee

‘CRISPRing the mouse genome: from simple editing to complex gene engineering’

Event Date: 
Thursday, April 26, 2018 - 10:00
Event Location: 
MSI Small Lecture Theatre
Host: 
Dr. Paul Davies
Event Speaker: 
Dr Evert-Jan Uringa
Institution: 
Scientific Program Management, Taconic Biosciences Oxford
Event Type: 
Seminar
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In this seminar, Dr Uringa will discuss Taconic’s capabilities in mouse model generation. The focus will be on the use of CRISPR technology. As an example of CRISPR/Cas9 versatility, he will describe its combination with gene targeting to introduce complex modifications in the mouse genome. Furthermore, he will discuss Easi-CRISPR (Efficient additions with ssDNA inserts-CRISPR), a targeting strategy in which long single-stranded DNA is injected with pre-assembled gRNA and Cas9 ribonucleoprotein complexes into mouse zygotes. Using easi-CRISPR Taconic can now efficiently generate Cre or fluorescent-protein knock-in animals.

 

In addition, Dr Uringa will introduce Taconic’s newest services, for example ExpressModel™. This service provides a mouse model up to the first experimental animal cohort, with the fastest and most predictable timeline in industry at a health status of choice.

 

 

 

Taconic’s Technological Advances and new Services

 

Rapidly changing technology is giving researchers increased choices and greater precision when it comes to building the optimal small animal model. As a fully-licensed provider of mouse and rat model generation services, Taconic stays current with the latest genetic and genomic technologies. In this seminar, Dr Uringa will discuss Taconic’s technological advances using CRISPR technology and the latest improvement in their services, including ExpressMODEL™.            

 

CRISPR/Cas9 is a great technology which is still developing. Taconic use it frequently for knockout and knock-in (point-mutations, epitope tags) models. In addition, Taconic use CRISPR technology as a complementary approach to gene targeting. As an example of CRISPR/Cas9 versatility, Dr Uringa will describe its combination with gene targeting to introduce complex modifications in the mouse genome. Most recently, Taconic have implemented Easi-CRISPR (Efficient additions with ssDNA inserts-CRISPR), a targeting strategy in which long single-stranded DNA is injected with pre-assembled gRNA and Cas9 ribonucleoprotein complexes into mouse zygotes. Using easi-CRISPR Taconic can now efficiently generate Cre or fluorescent-protein knock-in animals.

 

This year Taconic made some significant changes to their services by replacing germline breeding of founder and chimeric mice with In Vitro Fertilization (IVF) using QC-ed sperm. By doing so, they have removed the main causes of project delays: unproductive animals and animals with a low percentage of the wanted allele in the germline. Another benefit of doing the IVF using a founder or chimera is scalability of the deliverables. Taconic can now obtain 4-100 heterozygous animals in the first generation from a single animal, therefore bypassing at least one round of colony expansion, saving researchers 3-4 months on overall project timelines. This ExpressMODEL™ service, is a novel approach developed by Taconic Biosciences to reduce the timelines to experimental cohort delivery by 3-4 months.

 

Besides the subjects described above, Dr Uringa will be open to discussing anything which might be of interest to you.