Neutrophils are essential innate immune cells that combat invading pathogens using a variety of antimicrobial mechanisms. These include phagocytosis of microorganisms, release of antimicrobials by degranulation and the formation of neutrophil extracellular traps (NETs). Neutrophils acquire their antimicrobial machinery during their development in the bone marrow and enter circulation as short-lived, terminally differentiated cells. Due to their short life, mature neutrophils are not accessible to genetic manipulation, complicating research in these cells. I devised a genetic screening in neutrophil precursor cells and thereby identified novel regulators of neutrophil differentiation. Neutrophils expel NETs into the extracellular space through a unique cell death pathway. I therefore used the formation of NETs as an endpoint in a large chemical screen and demonstrated that the pore-forming protein gasdermin D is crucial for NET formation.