University of Dundee

"MASSIVELY PARALLEL FLUORESCENCE LIFETIME IMAGING MICROSCOPY TO INVESTIGATE CELLULAR DYNAMICS"

Event Date: 
Wednesday, November 14, 2018 - 12:00 to 13:00
Event Location: 
WTB Seminar Rooms
Event Speaker: 
Dr Simon Poland
Institution: 
Kings College London
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Event Type: 
Seminar

 

 

Abstract: Fluorescence lifetime imaging (FLIM) is a key fluorescence 

microscopy technique to map the environment and interaction of 

fluorescent probes. It can report on photophysical events that are 

difficult or impossible to observe by fluorescence intensity imaging, 

because FLIM is independent of the local fluorophore concentration and 

excitation intensity. For high precision FLIM, time-correlated single 

photon counting (TCSPC) is unparalleled in its measurement accuracy 

particularly for multi-exponential decays. Until recently, high speed 

FLIM could only be performed using modulated or time-gated image 

intensifier systems as TCSPC was fundamentally limited with respect to 

photon counting rate in implementations of laser scanning microscopy. In 

this talk the development of a massively parallelised multifocal FLIM 

laser scanning microscope will be discussed and its practical use will 

be shown in a number of time-critical applications including in-vivo 

imaging, diagnostics and histological screening.