University of Dundee

‘Serine ADP-ribosylation: a well-kept secret of PARP-1 and PARP-2’

Event Date: 
Thursday, August 29, 2019 - 14:00
Event Location: 
School of Life Sciences, MSI Small Lecture Theatre
Professor Dario Alessi FRS FRSE FMedSci
Event Speaker: 
Dr Juan José Bonfiglio
Max Planck Institute for Biology of Ageing
Event Type: 
MRC Protein Phosphorylation and Ubiquitylation Spotlight Seminar

Juan José Bonfiglio is currently working as a Marie Skłodowska-Curie supported Postdoc at the Max Planck Institutefor Biology of Ageing, Cologne, Germany, in the lab of Dr. Ivan Matic.


During his postdoc he has been working on one of the immediate responses upon DNA damage: the protein post-translational modification ADP-ribosylation and uncovered a new signaling pathway for this modification (Nat Chem Bio 2016) and described its biochemical basis by identifying its “writers” (Molecular Cell 2017) and “eraser” (eLife 2017). He has also shown that this is a widespread modification (Molecular Cell 2017). 


As there are no adequate tools to study ADP-ribosylation at the molecular level, Juan has developed a method to generate ADP-ribosylated peptides. He has been able to generate the first site-specific ADPr antibodies (unpublished results). 


Juan is visiting us to explore the possibility of setting up a laboratory in Dundee and will give a seminar about his research and future plans. 


Selected Publications


Interplay of Histone Marks with Serine ADP-Ribosylation. Bartlett E, Bonfiglio JJ, Prokhorova E, Colby T, Zobel F, Ahel I, Matic I. Cell Rep. 2018 Sep 25;24(13):3488-3502.e5. doi: 10.1016/j.celrep.2018.08.092.


Nonlocalized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides. Colby T, Bonfiglio JJ, Matic I. Methods Mol Biol. 2018;1813:255-269. doi: 10.1007/978-1-4939-8588-3_18.


Serine ADP-ribosylation reversal by the hydrolase ARH3. Fontana P, Bonfiglio JJ, Palazzo L, Bartlett E, Matic I, Ahel I. Elife. 2017 Jun 26;6. pii: e28533. doi: 10.7554/eLife.28533.


Mass spectrometry for serine ADP-ribosylation? Think o-glycosylation! Bonfiglio JJ, Colby T, Matic I. Nucleic Acids Res. 2017 Jun 20;45(11):6259-6264. doi: 10.1093/nar/gkx446.


Serine ADP-Ribosylation Depends on HPF1. Bonfiglio JJ, Fontana P, Zhang Q, Colby T, Gibbs-Seymour I, Atanassov I, Bartlett E, Zaja R, Ahel I, Matic I. Mol Cell. 2017 Mar 2;65(5):932-940.e6. doi: 10.1016/j.molcel.2017.01.003. Epub 2017 Feb 9.


Serine is a new target residue for endogenous ADP-ribosylation on histones. Leidecker O, Bonfiglio JJ, Colby T, Zhang Q, Atanassov I, Zaja R, Palazzo L, Stockum A, Ahel I, Matic I Nat Chem Biol. 2016 Dec;12(12):998-1000. doi: 10.1038/nchembio.2180. Epub 2016 Oct 10.